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Experimental analysis of moisture-resistant microbial penetration tester
2024/11/19
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The wet microbial penetration tester is used to determine the performance of the material in resisting the penetration of bacteria in the liquid when subjected to mechanical friction (shielding performance against the penetration of liquid-carried bacteria when subjected to mechanical friction). It is mainly used for medical surgical sheets, surgical gowns, and clean clothes. Standard Group (Hong Kong) Co., Ltd. is the manufacturer. Customers with needs are welcome to call for inquiries.
Applicable standards:
YY/T 0506.2, YY/T 0506.6-2009, EN ISO 22610
Main parameters:
1. Turntable speed: (60±1) rpm/min;
2. Test pressure: 3N±0.02N;
3. Outward wheel speed: 5~6rpm;
4. Timer setting range: 0~99.99min;
5. Total weight of inner and outer ring weights: 800g±1g.
Test experiment:
The test experiment mainly consists of 5 steps:
Step 1: Preparation of bacterial slices
Staphylococcus aureus ATCC29213 was cultured on tryptic soy agar at 36℃±1℃ for 18-24 hours, 2-3 colonies were inoculated into 3ml tryptic soy broth, and cultured at 36℃±1℃ for 18-24 hours. Diluted with peptone water 1:10 to a concentration of 1×104CFU/mL~4×104CFU/mL, and the bacterial suspension was counted.
Open the sterile bag take out the polyurethane film still attached to the IQ, and place the wettable polyurethane film of the bacterial material sheet facing up on the cleanliness plate. For ease of operation, fix the four corners of the bacterial material sheet to the plate with double-sided tape. Use the petri dish cover as a template to cut out a corresponding area on the bacterial film, apply 1.0ml of Staphylococcus aureus suspension on the area, and then dry the bacterial sheet at 56℃ for about 30 minutes. During the drying period, use a sterilized glass applicator to continue to apply the bacterial suspension on the bacterial film to evenly distribute the bacterial liquid. The bacterial sheet is used on the same day after it is prepared.
Step 2: State adjustment
If necessary, the specimen is state-adjusted according to GB6529, and the state can also be tested under standard normal room temperature conditions. The state adjustment method should be recorded in the test report.
Step 3: Test setting
Adjust the counterweight on the control rod so that the force applied to the agar by the test finger is 3N±0.02N. Place an agar petri dish on the turntable.
Step 4: Material application
Use the following count: Use a circular weight consisting of an inner and outer ring with a total weight of 800g±1g to apply a standard tension force to the material. Place the cylinder in the center of the inner ring, and then cover the test piece on the cylinder and the inner ring. Place the bacterial sheet with the stained surface facing down on the test piece after removing the attached paper. Cover the polyurethane film with a layer of HDPE film, push down the outer ring tightly, and firmly add the three layers of material between the two rings.
Step 5: Conduct the test
Gently place the above ring assembly on an agar culture dish with the lid removed, and let the steel ring hang freely outside the rotating disk. Place the test finger on the HDPE film on the inner side of the dish mouth so that the test piece can contact the agar surface. The test finger applies 3N pressure as specified above, and the instrument runs for 15 minutes. After 15 minutes, immediately remove the ring set and set it aside. Remove a culture dish from the rotating disk and put on the dish cover.
Immediately place the second culture dish and the ring set on the rotating disk.
Perform the above steps for the next four culture dishes with the same ring set assembly.
After the five culture dishes are tested, remove the bacterial sheet and discard it, turn the test piece over, and cover it with HDPE film upside down.
Operate on the sixth culture dish for 15 minutes to complete a parallel test group.
The remaining 4 specimens are also run in six culture dishes for 15 minutes each according to the above method, and each specimen uses a freshly prepared bacterial plate.
If there is liquid on the agar surface, place it on a clean bench to dry, cover each agar culture dish, and place it at 36℃±1℃ for 48 hours.
Count the number of Staphylococcus aureus colonies in each culture dish, and do not count the number of colonies within a 15-minute radius from the center of the culture dish.
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